Dataset Information

Epichromatin and chromomeres: a 'fuzzy' perspective.

ABSTRACT: 'Epichromatin', the surface of chromatin beneath the interphase nuclear envelope (NE) or at the surface of mitotic chromosomes, was discovered by immunostaining with a specific bivalent mouse monoclonal anti-nucleosome antibody (mAb PL2-6). 'Chromomeres', punctate chromatin particles approximately 200-300 nm in diameter, identified throughout the interphase chromatin and along mitotic chromosomes, were observed by immunostaining with the monovalent papain-derived Fab fragments of bivalent PL2-6. The specific target for PL2-6 appears to include the nucleosome acidic patch. Thus, within the epichromatin and chromomeric regions, this epitope is 'exposed'. Considering that histones possess unstructured 'tails' (i.e. intrinsically disordered peptide regions, IDPR), our perception of these chromatin regions becomes more 'fuzzy' (less defined). We suggest that epichromatin cationic tails facilitate interactions with anionic components of NE membranes. We also suggest that the unstructured histone tails (especially, histone H1 tails), with their presumed promiscuous binding, establish multivalent binding that stabilizes each chromomere as a unit of chromatin higher order structure. We propose an 'unstructured stability' hypothesis, which postulates that the stability of epichromatin and chromomeres (as well as other nuclear chromatin structures) is a consequence of the collective contributions of numerous weak histone IDPR binding interactions arising from the multivalent nucleosome, analogous to antibody avidity.

SUBMITTER: Olins DE

PROVIDER: S-EPMC6030114 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Epichromatin and chromomeres: a 'fuzzy' perspective.

Olins Donald E DE Olins Ada L AL

Open biology 20180601 6

'Epichromatin', the surface of chromatin beneath the interphase nuclear envelope (NE) or at the surface of mitotic chromosomes, was discovered by immunostaining with a specific <i>bivalent</i> mouse monoclonal anti-nucleosome antibody (mAb PL2-6). 'Chromomeres', punctate chromatin particles approximately 200-300 nm in diameter, identified throughout the interphase chromatin and along mitotic chromosomes, were observed by immunostaining with the <i>monovalent</i> papain-derived Fab fragments of b ...[more]

PMID: 29875200

Similar Datasets

Project description:The present paper explores nonnative (L2) phonological encoding of lexical entries and dissociates the difficulties associated with L2 phonological and phonolexical encoding by focusing on similarly sounding L2 words that are not differentiated by difficult phonological contrasts. We test two main claims of the fuzzy lexicon hypothesis: (1) L2 fuzzy phonolexical representations are not fully specified and lack details at both phonological and phonolexical levels of representation (Experiment 1); and (2) fuzzy phonolexical representations can lead to establishing incorrect form-to-meaning mappings (Experiment 2). The Russian-English Translation Judgment Task (Experiment 1, TJT) explores how the degree of phonolexical similarity between a word and its lexical competitor affects lexical access of Russian words. Words with smaller phonolexical distance (e.g., parent-parrot) show longer reaction times and lower accuracy compared to words with a larger phonolexical distance (e.g., parent-parchment) in lower-proficiency nonnative speakers, and, to a lesser degree, higher-proficiency speakers. This points to a lack of detail in nonnative phonolexical representations necessary for efficient lexical access. The Russian Pseudo-Semantic Priming task (Experiment 2, PSP) addresses the vulnerability of form-to-meaning mappings as a consequence of fuzzy phonolexical representations in L2. We primed the target with a word semantically related to its phonological competitor, or a potentially confusable word. The findings of Experiment 2 extend the results of Experiment 1 that, unlike native speakers, nonnative speakers do not properly encode phonolexical information. As a result, they are prone to access an incorrect lexical representation of a competitor word, as indicated by a slowdown in the judgments to confusable words. The study provides evidence that fuzzy phonolexical representations result in unfaithful form-to-meaning mappings, which lead to retrieval of incorrect semantic content. The results of the study are in line with existing research in support of less detailed L2 phonolexical representations, and extend the findings to show that the fuzziness of phonolexical representations can arise even when confusable words are not differentiated by difficult phonological contrasts.

Dataset Information

Epichromatin and chromomeres: a 'fuzzy' perspective.

Publications

Epichromatin and chromomeres: a 'fuzzy' perspective.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets