Ontology highlight
ABSTRACT:
SUBMITTER: Lemmens B
PROVIDER: S-EPMC6039720 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Lemmens Bennie B Hegarat Nadia N Akopyan Karen K Sala-Gaston Joan J Bartek Jiri J Hochegger Helfrid H Lindqvist Arne A
Molecular cell 20180628 1
To maintain genome stability, cells need to replicate their DNA before dividing. Upon completion of bulk DNA synthesis, the mitotic kinases CDK1 and PLK1 become active and drive entry into mitosis. Here, we have tested the hypothesis that DNA replication determines the timing of mitotic kinase activation. Using an optimized double-degron system, together with kinase inhibitors to enforce tight inhibition of key proteins, we find that human cells unable to initiate DNA replication prematurely ent ...[more]