Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease.

ABSTRACT: Background:The CARD family plays an important role in innate immune response by the activation of NF-?B. The aim of this study was to determine the gene expression and to enumerate the protein-expressing cells of some members of the CARD family (CARD9, CARD10, CARD11, CARD14 and CARD15) in patients with IBD and normal controls without colonic inflammation. Methods:We included 48 UC patients, 10 Crohn's disease (CD) patients and 18 non-inflamed controls. Gene expression was performed by RT-PCR and protein expression by immunohistochemistry. CARD-expressing cells were assessed by estimating the positively staining cells and reported as the percentage. Results:The CARD9 and CARD10 gene expression was significantly higher in UC groups compared with CD (P?< 0.001). CARD11 had lower gene expression in UC than in CD patients (P?< 0.001). CARD14 gene expression was higher in the group with active UC compared to non-inflamed controls (P?< 0.001). The low expression of CARD14 gene was associated with a benign clinical course of UC, characterized by initial activity followed by long-term remission longer than 5 years (P?= 0.01, OR?=?0.07, 95%CI:0.007-0.70). CARD15 gene expression was lower in UC patients versus CD (P?= 0.004). CARD9 protein expression was detected in inflammatory infiltrates; CARD14 in parenchymal cells, while CARD15 in inflammatory and parenchymal cells. CARD9-, CARD14- and CARD15?-?expressing cells were significantly higher in patients with active UC versus non-inflamed controls (P?< 0.05). Conclusion:The CARD family is involved in the inflammatory process and might be involved in the IBD pathophysiology.

SUBMITTER: Yamamoto-Furusho JK 

PROVIDER: S-EPMC6042317 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature