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PStab: prediction of stable mutants, unfolding curves, stability maps and protein electrostatic frustration.

ABSTRACT: Summary:We present a web-server for rapid prediction of changes in protein stabilities over a range of temperatures and experimental conditions upon single- or multiple-point substitutions of charged residues. Potential mutants are identified by a charge-shuffling procedure while the stability changes (i.e. an unfolding curve) are predicted employing an ensemble-based statistical-mechanical model. We expect this server to be a simple yet detailed tool for engineering stabilities, identifying electrostatically frustrated residues, generating local stability maps and in constructing fitness landscapes. Availability and implementation:The web-server is freely available at http://pbl.biotech.iitm.ac.in/pStab and supports recent versions of all major browsers. Contact:athi@iitm.ac.in. Supplementary information:Supplementary data are available at Bioinformatics online.

SUBMITTER: Gopi S

PROVIDER: S-EPMC6049017 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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pStab: prediction of stable mutants, unfolding curves, stability maps and protein electrostatic frustration.

Gopi Soundhararajan S Devanshu Devanshu D Krishna Praveen P Naganathan Athi N AN

Bioinformatics (Oxford, England) 20180301 5

<h4>Summary</h4>We present a web-server for rapid prediction of changes in protein stabilities over a range of temperatures and experimental conditions upon single- or multiple-point substitutions of charged residues. Potential mutants are identified by a charge-shuffling procedure while the stability changes (i.e. an unfolding curve) are predicted employing an ensemble-based statistical-mechanical model. We expect this server to be a simple yet detailed tool for engineering stabilities, identif ...[more]

PMID: 29092002

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