Proteomics

Dataset Information

0

Surveyance of apparent proteome unfolding curves via thiol labelling


ABSTRACT: Census of apparent proteome unfolding curves following urea denaturation in cell lysate using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Dezerae Cox  

LAB HEAD: Danny M. Hatters

PROVIDER: PXD022640 | Pride | 2022-05-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180309_DezeraeC_DC074-DC086.msf Msf
180309_DezeraeC_DC074-DC086.pdResult Other
180309_DezeraeC_DC074.raw Raw
180309_DezeraeC_DC075.raw Raw
180309_DezeraeC_DC076.raw Raw
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Publications

Hidden information on protein function in censuses of proteome foldedness.

Cox Dezerae D   Ang Ching-Seng CS   Nillegoda Nadinath B NB   Reid Gavin E GE   Hatters Danny M DM  

Nature communications 20220414 1


Methods that assay protein foldedness with proteomics have generated censuses of apparent protein folding stabilities in biological milieu. However, different censuses poorly correlate with each other. Here, we show that the reason for this is that methods targeting foldedness through monitoring amino acid sidechain reactivity also detect changes in conformation and ligand binding, which can be a substantial fraction of the data. We show that the reactivity of only one quarter of cysteine or met  ...[more]

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