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Down-Regulation of PpBGAL10 and PpBGAL16 Delays Fruit Softening in Peach by Reducing Polygalacturonase and Pectin Methylesterase Activity.


ABSTRACT: ?-galactosidases are cell wall hydrolases that play an important role in fruit softening. However, PpBGALs mechanism impacting on ethylene-dependent peach fruit softening was still unclear. In this study, we found that PpBGAL4, -6, -8, -10, -16, and -17 may be required for ethylene-dependent peach softening and PpBGAL10, -16 may make a main contribution to it among 17 PpBGALs. Utilization of virus-induced gene silencing (VIGS) showed that fruits were firmer than those of the control at 4 and 6 days after harvest (DAH) when PpBGAL10 and PpBGAL16 expression was down-regulated. Suppression of PpBGAL10 and PpBGAL16 expression also reduced PpPG21 and PpPME3 transcription, and polygalacturonase (PG) and pectinmethylesterases (PME) activity. Overall, total cell wall material and protopectin slowly declined, water-soluble pectin slowly increased, and cellulose and hemicellulose was altered significantly at 4 DAH, relative to control fruit. In addition, PpACO1 expression and ethylene production were also suppressed at 4 DAH because of inhibiting PpBGAL10 and PpBGAL16 expression. These results suggested that down-regulation of PpBGAL10 and PpBGAL16 expression delays peach fruit softening by decreasing PG and PME activity, which inhibits cell wall degradation and ethylene production.

SUBMITTER: Liu H 

PROVIDER: S-EPMC6050397 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Down-Regulation of <i>PpBGAL10</i> and <i>PpBGAL16</i> Delays Fruit Softening in Peach by Reducing Polygalacturonase and Pectin Methylesterase Activity.

Liu Hangkong H   Qian Ming M   Song Chunhui C   Li Jinjin J   Zhao Caiping C   Li Guofang G   Wang Anzhu A   Han Mingyu M  

Frontiers in plant science 20180711


β-galactosidases are cell wall hydrolases that play an important role in fruit softening. However, <i>PpBGAL</i>s mechanism impacting on ethylene-dependent peach fruit softening was still unclear. In this study, we found that <i>PpBGAL4, -6, -8, -10, -16</i>, and -<i>17</i> may be required for ethylene-dependent peach softening and <i>PpBGAL10</i>, -<i>16</i> may make a main contribution to it among 17 <i>PpBGALs</i>. Utilization of virus-induced gene silencing (VIGS) showed that fruits were fir  ...[more]

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