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Postharvest Treatment of Hydrogen Sulfide Delays the Softening of Chilean Strawberry Fruit by Downregulating the Expression of Key Genes Involved in Pectin Catabolism.

ABSTRACT: Hydrogen sulfide (H₂S) plays several physiological roles in plants. Despite the evidence, the role of H₂S on cell wall disassembly and its implications on fleshy fruit firmness remains unknown. In this work, the effect of H₂S treatment on the shelf-life, cell wall polymers and cell wall modifying-related gene expression of Chilean strawberry (Fragaria chiloensis) fruit was tested during postharvest storage. The treatment with H₂S prolonged the shelf-life of fruit by an effect of optimal dose. Fruit treated with 0.2 mM H₂S maintained significantly higher fruit firmness than non-treated fruit, reducing its decay and tripling its shelf-life. Additionally, H₂S treatment delays pectin degradation throughout the storage period and significantly downregulated the expression of genes encoding for pectinases, such as polygalacturonase, pectate lyase, and expansin. This evidence suggests that H₂S as a gasotransmitter prolongs the post-harvest shelf-life of the fruit and prevents its fast softening rate by a downregulation of the expression of key pectinase genes, which leads to a decreased pectin degradation.

SUBMITTER: Molinett SA

PROVIDER: S-EPMC8469075 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening.

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2021-01-21 | E-MTAB-8736 | biostudies-arrayexpress

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Project description:Genetic improvement of tomato by targeted control of fruit softening. Uluisik et al. Nature Biotechnology (2016) doi:10.1038/nbt.3602

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| S-EPMC9752004 | biostudies-literature

Genomic Prediction of Strawberry Resistance to Postharvest Fruit Decay Caused by the Fungal Pathogen Botrytis cinerea.

Project description:Gray mold, a disease of strawberry (Fragaria ×ananassa) caused by the ubiquitous necrotroph Botrytis cinerea, renders fruit unmarketable and causes economic losses in the postharvest supply chain. To explore the feasibility of selecting for increased resistance to gray mold, we undertook genetic and genomic prediction studies in strawberry populations segregating for fruit quality and shelf life traits hypothesized to pleiotropically affect susceptibility. As predicted, resistance to gray mold was heritable but quantitative and genetically complex. While every individual was susceptible, the speed of symptom progression and severity differed. Narrow-sense heritability ranged from 0.38-0.71 for lesion diameter (LD) and 0.39-0.44 for speed of emergence of external mycelium (EM). Even though significant additive genetic variation was observed for LD and EM, the phenotypic ranges were comparatively narrow and genome-wide analyses did not identify any large effect loci. Genomic selection accuracy ranged from 0.28-0.59 for LD and 0.37-0.47 for EM. Additive genetic correlations between fruit quality and gray mold resistance traits were consistent with prevailing hypotheses: LD decreased as titratable acidity increased, whereas EM increased as soluble solid content decreased and firmness increased. We concluded that phenotypic and genomic selection could be effective for reducing LD and increasing EM, especially in long shelf life populations, but that a significant fraction of the genetic variation for resistance to gray mold was caused by the pleiotropic effects of fruit quality traits that differ among market and shelf life classes.

| S-EPMC8728004 | biostudies-literature

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