Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides.
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ABSTRACT:
SUBMITTER: Khatami A
PROVIDER: S-EPMC6051451 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides.
Khatami Ameneh A Randis Tara M TM Chamby Anna A Hooven Thomas A TA Gegick Margaret M Suzman Evan E A'Hearn-Thomas Brady B Steenhoff Andrew P AP Ratner Adam J AJ
Open forum infectious diseases 20180707 7
Group B <i>Streptococcus</i> (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including <i>sip</i>. We demonstrate suboptimal sensitivity of the existing <i>sip</i> PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes. ...[more]
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