Distinct ways of G:U recognition by conserved tRNA binding motifs.

ABSTRACT: Throughout three domains of life, alanyl-tRNA synthetases (AlaRSs) recognize a G3:U70 base pair in the acceptor stem of tRNA^Ala as the major identity determinant of tRNA^Ala The crystal structure of the archaeon Archaeoglobus fulgidus AlaRS in complex with tRNA^Ala provided the basis for G3:U70 recognition with residues (Asp and Asn) that are conserved in the three domains [Naganuma M, et al. (2014) Nature 510:507-511]. The recognition mode is unprecedented, with specific accommodation of the dyad asymmetry of the G:U wobble pair and exclusion of the dyad symmetry of a Watson-Crick pair. With this conserved mode, specificity is based more on "fit" than on direct recognition of specific atomic groups. Here, we show that, in contrast to the archae

SUBMITTER: Chong YE 

PROVIDER: S-EPMC6055181 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature