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Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

ABSTRACT: The degradation of TNFM-NM-1 mRNA is critical for restricting TNFM-NM-1 synthesis and involves a constitutive decay element (CDE) in the 3M-bM-^@M-^Y untranslated region of the mRNA. We demonstrate that the active CDE is a stem-loop RNA motif that is recognized by the RNA-binding protein Roquin. Deep sequencing of Roquin-associated mRNAs identified CDE-containing mRNAs as the primary targets of Roquin on a transcriptome-wide scale. Expression profiles of LPS treated Raw264.7 cells (input), after Roquin IP or control IP were generated by deep sequencing, in three biological replicates, using Illumina HiSeq 2000.

ORGANISM(S): Mus musculus

SUBMITTER: Georg Stoecklin

PROVIDER: E-GEOD-44775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Leppek Kathrin K Schott Johanna J Reitter Sonja S Poetz Fabian F Hammond Ming C MC Stoecklin Georg G

Cell 20130501 4

Tumor necrosis factor-α (TNF-α) is the most potent proinflammatory cytokine in mammals. The degradation of TNF-α mRNA is critical for restricting TNF-α synthesis and involves a constitutive decay element (CDE) in the 3' UTR of the mRNA. Here, we demonstrate that the CDE folds into an RNA stem-loop motif that is specifically recognized by Roquin and Roquin2. Binding of Roquin initiates degradation of TNF-α mRNA and limits TNF-α production in macrophages. Roquin proteins promote mRNA degradation b ...[more]

PMID: 23663784

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