Ontology highlight
ABSTRACT:
SUBMITTER: Miura H
PROVIDER: S-EPMC6058056 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Miura Hiromi H Quadros Rolen M RM Gurumurthy Channabasavaiah B CB Ohtsuka Masato M
Nature protocols 20171221 1
CRISPR/Cas9-based genome editing can easily generate knockout mouse models by disrupting the gene sequence, but its efficiency for creating models that require either insertion of exogenous DNA (knock-in) or replacement of genomic segments is very poor. The majority of mouse models used in research involve knock-in (reporters or recombinases) or gene replacement (e.g., conditional knockout alleles containing exons flanked by LoxP sites). A few methods for creating such models have been reported ...[more]