Ontology highlight
ABSTRACT:
SUBMITTER: Potapov V
PROVIDER: S-EPMC6061786 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Potapov Vladimir V Ong Jennifer L JL Langhorst Bradley W BW Bilotti Katharina K Cahoon Dan D Canton Barry B Knight Thomas F TF Evans Thomas C TC Lohman Gregory J S GJS
Nucleic acids research 20180701 13
DNA ligases are key enzymes in molecular and synthetic biology that catalyze the joining of breaks in duplex DNA and the end-joining of DNA fragments. Ligation fidelity (discrimination against the ligation of substrates containing mismatched base pairs) and bias (preferential ligation of particular sequences over others) have been well-studied in the context of nick ligation. However, almost no data exist for fidelity and bias in end-joining ligation contexts. In this study, we applied Pacific B ...[more]