Dataset Information

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations.

ABSTRACT: Monitoring the physicochemical characteristics of nanoparticles following internalisation by cells is a vital step in understanding their biological impact and toxicity. Here, the feasibility of a methodology utilising gentle enzymatic lysis of cells containing internalised particles and direct analysis of the lysates for the particle size, agglomeration state and concentration, is investigated. It is demonstrated that following internalisation, all types of studied silica particles partially agglomerate/aggregate, with the degree and rate of the observed transformation closely correlated with the initial particle surface chemistry. Several different particle populations are noted and characterised in terms of their size and concentration. Good agreement between different complementary techniques is reached in terms of the average particle diameter. Particle concentration is determined here with techniques capable of mass and number-based measurements, with limitations of approaches utilising signal conversion to equivalent particle numbers identified and discussed.

SUBMITTER: Bartczak D

PROVIDER: S-EPMC6062163 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations.

Bartczak Dorota D Davies Julie J Gollwitzer Christian C Krumrey Michael M Goenaga-Infante Heidi H

Toxicology research 20180123 2

Monitoring the physicochemical characteristics of nanoparticles following internalisation by cells is a vital step in understanding their biological impact and toxicity. Here, the feasibility of a methodology utilising gentle enzymatic lysis of cells containing internalised particles and direct analysis of the lysates for the particle size, agglomeration state and concentration, is investigated. It is demonstrated that following internalisation, all types of studied silica particles partially ag ...[more]

PMID: 30090572

Similar Datasets

Project description:Despite increasing reports of using silica nanoparticles (SNPs) for controlled drug delivery applications, their long-term toxicity profile following intravenous administration remains unexplored. Herein, we investigated the acute (10-day) and subchronic (60-day and 180-day) toxicity of nonporous SNPs of approximately 50 nm (Stöber SNPs50) and approximately 500 nm in diameter (Stöber SNPs500), and mesoporous SNPs of approximately 500 nm in diameter (MSNPs500) upon single-dose intravenous injection into male and female immune-competent inbred BALB/c mice. The Maximum Tolerated Dose (MTD) of the particles was determined 10 days post-injection. The MTD of SNPs was administered and toxicity evaluated over 60 and 180 days. Results demonstrate that Stöber SNPs50 exhibit systemic toxicity with MTD of 103 ± 11 mg.kg-1 for female and 100 ± 6 mg.kg-1 for male mice, respectively. Toxicity was alleviated by increasing the size of the particles (Stöber SNPs500). MTD values of 303 ± 4 mg.kg-1 for female and 300 ± 13 mg.kg-1 for male were observed for Stöber SNPs500. Mesoporous SNPs500 showed considerable systemic sex-related toxicity, with MTDs ranging from 40 ± 2 mg.kg-1 to 95 ± 2 mg.kg-1 for male and female mice, respectively. Studies of SNPs showed blood toxicity as a function of physiochemical properties such as significant differences in the mean corpuscular hemoglobin (MCHC) and platelet number at day 10 and white blood cell count at day 60. Histological examination also showed size-, porosity- and time-dependent tissue toxicity. Stöber SNPs500 caused major toxic effects such as lung thrombosis, cardiac wall fibrosis and calcifications, brain infarctions with necrotizing inflammatory response, infiltrate, retinal injuries with calcification and focal gliosis, renal parenchymal damage and liver lobular inflammation dependent on the dose and time of exposure. However, tissue toxicity and accumulation of SNPs in liver observed at day 10 was greater than at day 60 and much greater than at day 180. In contrast, a dramatic increase in cytokine levels was observed at day 60. Despite the relatively high doses, SNPs did not cause subchronic toxicity at day 180 after single-dose intravenous injection. However, they showed distinct differences in the 60 day in vivo subchronic toxicity and inflammation profile as a function of surface area and size.

Dataset Information

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations.

Publications

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets