Ontology highlight
ABSTRACT:
SUBMITTER: Tycko J
PROVIDER: S-EPMC6063963 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Tycko Josh J Barrera Luis A LA Huston Nicholas C NC Friedland Ari E AE Wu Xuebing X Gootenberg Jonathan S JS Abudayyeh Omar O OO Myer Vic E VE Wilson Christopher J CJ Hsu Patrick D PD
Nature communications 20180727 1
Therapeutic genome editing with Staphylococcus aureus Cas9 (SaCas9) requires a rigorous understanding of its potential off-target activity in the human genome. Here we report a high-throughput screening approach to measure SaCas9 genome editing variation in human cells across a large repertoire of 88,692 single guide RNAs (sgRNAs) paired with matched or mismatched target sites in a synthetic cassette. We incorporate randomized barcodes that enable whitelisting of correctly synthesized molecules ...[more]