Ontology highlight
ABSTRACT:
SUBMITTER: Casini A
PROVIDER: S-EPMC6066108 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Casini Antonio A Olivieri Michele M Petris Gianluca G Montagna Claudia C Reginato Giordano G Maule Giulia G Lorenzin Francesca F Prandi Davide D Romanel Alessandro A Demichelis Francesca F Inga Alberto A Cereseto Anna A
Nature biotechnology 20180129 3
Despite the utility of CRISPR-Cas9 nucleases for genome editing, the potential for off-target activity limits their application, especially for therapeutic purposes. We developed a yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous evaluation of on- and off-target activity. We screened a library of SpCas9 variants carrying random mutations in the REC3 domain and identified mutations that increased editing accuracy while maintaining edi ...[more]