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An RNA-Binding Multimer Specifies Nematode Sperm Fate.

ABSTRACT: FOG-3 is a master regulator of sperm fate in Caenorhabditis elegans and homologous to Tob/BTG proteins, which in mammals are monomeric adaptors that recruit enzymes to RNA binding proteins. Here, we determine the FOG-3 crystal structure and in vitro demonstrate that FOG-3 forms dimers that can multimerize. The FOG-3 multimeric structure has a basic surface potential, suggestive of binding nucleic acid. Consistent with that prediction, FOG-3 binds directly to nearly 1,000 RNAs in nematode spermatogenic germ cells. Most binding is to the 3' UTR, and most targets (94%) are oogenic mRNAs, even though assayed in spermatogenic cells. When tethered to a reporter mRNA, FOG-3 represses its expression. Together these findings elucidate the molecular mechanism of sperm fate specification and reveal the evolution of a protein from monomeric to multimeric form with acquisition of a distinct mode of mRNA repression.

SUBMITTER: Aoki ST

PROVIDER: S-EPMC6066285 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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An RNA-Binding Multimer Specifies Nematode Sperm Fate.

Aoki Scott T ST Porter Douglas F DF Prasad Aman A Wickens Marvin M Bingman Craig A CA Kimble Judith J

Cell reports 20180601 13

FOG-3 is a master regulator of sperm fate in Caenorhabditis elegans and homologous to Tob/BTG proteins, which in mammals are monomeric adaptors that recruit enzymes to RNA binding proteins. Here, we determine the FOG-3 crystal structure and in vitro demonstrate that FOG-3 forms dimers that can multimerize. The FOG-3 multimeric structure has a basic surface potential, suggestive of binding nucleic acid. Consistent with that prediction, FOG-3 binds directly to nearly 1,000 RNAs in nematode spermat ...[more]

PMID: 29949762

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An RNA binding polymer specifies nematode sperm fate

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