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An RNA binding polymer specifies nematode sperm fate


ABSTRACT: Metazoan gamete development into sperm or oocytes requires specific programming cues. Many of these cues function through conserved proteins to regulate gene expression. In C. elegans, two proteins are the terminal regulators of the genetic regulatory network controlling sperm fate: FOG-1, a cytoplasmic polyadenylation element binding protein (CPEB), and FOG-3, a predicted Tob/BTG protein. Loss of either gene causes hermaphrodites or male germ cells to switch from making sperm to making oocytes. Characterized Tob/BTG proteins function as adaptor proteins, linking – in certain contexts – deadenylases to mRNA via CPEB RNA motifs. Through crosslinking and sequencing, we confirmed FOG-3 binds to RNA directly and observed a preference for mRNA 3’UTRs. Our results bring together prior genetics and molecular biology data to propose a new molecular function in germ cell specification with a Tob protein. We discuss the potential for FOG-3 to function as an mRNA packaging protein to inhibit target genes, as well as the potential for the existence of other hidden protein polymers in biology.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE76521 | GEO | 2016/12/30

SECONDARY ACCESSION(S): PRJNA307605

REPOSITORIES: GEO

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