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Collagen fiber recruitment: A microstructural basis for the nonlinear response of the posterior pole of the eye to increases in intraocular pressure.

ABSTRACT: Our goal was to quantify and characterize how the collagen fiber crimp waviness of the lamina cribrosa (LC) and peripapillary sclera (PPS) changes with intraocular pressure (IOP). Thirteen sheep (ovine) eyes were immersion and perfusion fixed while maintaining IOP at 0, 10, 15, 20, or 50?mmHg. Coronal optic nerve head (ONH) sections (30?µm) were imaged with polarized light microscopy (PLM) and analyzed for collagen fiber orientation and waviness (SD of fiber orientation). In the LC, the waviness of every LC beam was measured. In the PPS, at least 900 collagen bundles were measured per eye. Using the waviness at 50?mmHg IOP, we defined tissue-specific thresholds to determine the fraction of loaded or recruited fibers. We found that fiber waviness decreased with IOP (P?

SUBMITTER: Jan NJ

PROVIDER: S-EPMC6071322 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Collagen fiber recruitment: A microstructural basis for the nonlinear response of the posterior pole of the eye to increases in intraocular pressure.

Jan Ning-Jiun NJ Sigal Ian A IA

Acta biomaterialia 20180321

Our goal was to quantify and characterize how the collagen fiber crimp waviness of the lamina cribrosa (LC) and peripapillary sclera (PPS) changes with intraocular pressure (IOP). Thirteen sheep (ovine) eyes were immersion and perfusion fixed while maintaining IOP at 0, 10, 15, 20, or 50 mmHg. Coronal optic nerve head (ONH) sections (30 µm) were imaged with polarized light microscopy (PLM) and analyzed for collagen fiber orientation and waviness (SD of fiber orientation). In the LC, the waviness ...[more]

PMID: 29574185

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