Ontology highlight
ABSTRACT:
SUBMITTER: Morris KL
PROVIDER: S-EPMC6091687 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Morris Kyle L KL Buffalo Cosmo Z CZ Stürzel Christina M CM Heusinger Elena E Kirchhoff Frank F Ren Xuefeng X Hurley James H JH
Cell 20180701 3
The HIV accessory protein Nef counteracts immune defenses by subverting coated vesicle pathways. The 3.7 Å cryo-EM structure of a closed trimer of the clathrin adaptor AP-1, the small GTPase Arf1, HIV-1 Nef, and the cytosolic tail of the restriction factor tetherin suggested a mechanism for inactivating tetherin by Golgi retention. The 4.3 Å structure of a mutant Nef-induced dimer of AP-1 showed how the closed trimer is regulated by the dileucine loop of Nef. HDX-MS and mutational analysis were ...[more]