A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex.
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ABSTRACT:
SUBMITTER: Keaveney MK
PROVIDER: S-EPMC6095194 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex.
Keaveney Marianna K MK Tseng Hua-An HA Ta Tina L TL Gritton Howard J HJ Man Heng-Ye HY Han Xue X
Cell reports 20180701 2
More specific and broadly applicable viral gene-targeting tools for labeling neuron subtypes are needed to advance neuroscience research, especially in rodent transgenic disease models and genetically intractable species. Here, we develop a viral vector that restricts transgene expression to GABAergic interneurons in the rodent neocortex by exploiting endogenous microRNA regulation. Our interneuron-targeting, microRNA-guided neuron tag, "GABA mAGNET," achieves >95% interneuron selective labeling ...[more]
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