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Approaching cellular resolution and reliable identification in mass spectrometry imaging of tryptic peptides.

ABSTRACT: On-tissue digestion has become the preferred method to identify proteins in mass spectrometry (MS) imaging. In this study, we report advances in data acquisition and protein identification for MS imaging after on-tissue digestion. Tryptic peptides in a coronal mouse brain section were measured at 50 ?m pixel size and revealed detailed histological structures, e.g., the ependyma (consisting of one to two cell layers), which was confirmed by H&E staining. This demonstrates that MS imaging of tryptic peptides at or close to cellular resolution is within reach. We also describe a detailed identification workflow which resulted in the identification of 99 proteins (with 435 corresponding peptides), based on comparison with LC-MS/MS data and in silico digest. These results were obtained with stringent parameters, including high mass accuracy in imaging mode (RSME

SUBMITTER: Huber K

PROVIDER: S-EPMC6096711 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Approaching cellular resolution and reliable identification in mass spectrometry imaging of tryptic peptides.

Huber Katharina K Khamehgir-Silz Pegah P Schramm Thorsten T Gorshkov Vladimir V Spengler Bernhard B Römpp Andreas A

Analytical and bioanalytical chemistry 20180801 23

On-tissue digestion has become the preferred method to identify proteins in mass spectrometry (MS) imaging. In this study, we report advances in data acquisition and protein identification for MS imaging after on-tissue digestion. Tryptic peptides in a coronal mouse brain section were measured at 50 μm pixel size and revealed detailed histological structures, e.g., the ependyma (consisting of one to two cell layers), which was confirmed by H&E staining. This demonstrates that MS imaging of trypt ...[more]

PMID: 30066193

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