Ontology highlight
ABSTRACT:
SUBMITTER: Mason DM
PROVIDER: S-EPMC6101513 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Mason Derek M DM Weber Cédric R CR Parola Cristina C Meng Simon M SM Greiff Victor V Kelton William J WJ Reddy Sai T ST
Nucleic acids research 20180801 14
Antibody engineering is often performed to improve therapeutic properties by directed evolution, usually by high-throughput screening of phage or yeast display libraries. Engineering antibodies in mammalian cells offer advantages associated with expression in their final therapeutic format (full-length glycosylated IgG); however, the inability to express large and diverse libraries severely limits their potential throughput. To address this limitation, we have developed homology-directed mutagen ...[more]