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Autophagy is required for proper meiosis of porcine oocytes maturing in vitro.

ABSTRACT: Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44?h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown and treatment with the autophagy inhibitor, LY294002, during maturation of porcine oocytes in vitro impaired polar body extrusion, disturbed mitochondrial function, triggered the DNA damage response, and induced early apoptosis in porcine oocytes. Autophagy inhibition during oocyte maturation also impaired the further developmental potential of porcine oocytes. These results indicate that autophagy is required for the in vitro maturation of porcine oocytes.

SUBMITTER: Shen XH

PROVIDER: S-EPMC6105682 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Autophagy is required for proper meiosis of porcine oocytes maturing in vitro.

Shen Xing-Hui XH Jin Yong-Xun YX Liang Shuang S Kwon Jeong-Woo JW Zhu Ji-Wei JW Lei Lei L Lei Lei L Kim Nam-Hyung NH

Scientific reports 20180822 1

Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44 h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown ...[more]

PMID: 30135500

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