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Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like ?-Lactamase.

ABSTRACT: A multidrug-resistant Klebsiella pneumoniae 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded blaOXA-48-like gene, named blaOXA-519 The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS1R insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced ?-lactam-hydrolysis profile, except those for ertapenem and meropenem, which were increased. Thus, detection of OXA-519 producers using biochemical tests that monitor imipenem hydrolysis will be difficult.

SUBMITTER: Dabos L

PROVIDER: S-EPMC6105830 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase.

Dabos Laura L Bogaerts Pierre P Bonnin Remy A RA Zavala Agustin A Sacré Pierre P Iorga Bogdan I BI Huang Daniel T DT Glupczynski Youri Y Naas Thierry T

Antimicrobial agents and chemotherapy 20180727 8

A multidrug-resistant Klebsiella pneumoniae 1210 isolate with reduced carbapenem susceptibility revealed the presence of a novel plasmid-encoded blaOXA-48-like gene, named blaOXA-519 The 60.7-kb plasmid (pOXA-519) was similar to the IncL-OXA-48 prototypical plasmid except for a ca. 2-kb deletion due to an IS1R insertion. OXA-519 differed from OXA-48 by a Val120Leu substitution, which resulted in an overall reduced β-lactam-hydrolysis profile, exc ...[more]

PMID: 29866857

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