Preliminary study showing no association between G238A (rs361525) tumor necrosis factor-? (TNF-?) gene polymorphism and its serum level, hormonal and biochemical aspects of polycystic ovary syndrome.

ABSTRACT: BACKGROUND:Polycystic ovary syndrome (PCOS) is the main cause of female infertility. Interactions among genetic, biochemical, and immunological factors can affect the pathogenesis of PCOS. As a proinflammatory cytokine, tumor necrosis factor-? (TNF-?) plays an important role in this regard. The present study aimed to evaluate the association of the rs361525 gene single-nucleotide polymorphism (SNP) and TNF-? serum levels with the hormonal and biochemical characteristics of PCOS in Iranian individuals. METHODS:The SNP rs361525 in the TNF-? gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in a total of 111 PCOS patients and 105 healthy females. Serum levels of TNF-?, lipid and hormone profiles, and biochemical factors were measured using enzyme-linked immunosorbent assay (ELISA) and calorimetric methods, as appropriate. RESULTS:The TNF-? serum level was higher in women with PCOS compared with the control group (p?? 0.05). Higher levels and significant differences were found for the HOMA factor, luteinizing hormone/follicle-stimulating hormone (LH/FSH), testosterone, and body mass index (BMI) in the PCOS group compared with the control group (p?

SUBMITTER: Kordestani F 

PROVIDER: S-EPMC6106732 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature