Dataset Information

Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal.

ABSTRACT: This work studied the microbial community in partial nitritation and complete nitrification processes, which were applied to treat the low Carbon Nitrogen ratio wastewater. The phospholipid fatty acid and quantitative PCR analysis showed that the sludge circulating ratio of 75% resulted in a good microbial growth and a higher abundance of ammonia oxidizing bacteria relative to the nitrite oxidizing bacteria. The Betaproteobacteria were observed to compose the most abundant sludge bacterial groups in the two processes, based on phylogenetic analysis. The phylogenetic analysis of both 16S rRNA and amoA gene indicated that the Nitrosomonas sp. were the dominant ammonia oxidizing bacteria in the partial nitritation process. The relative abundance of nitrite oxidizing bacteria, such as Nitrobacter sp. and Nitrospira sp., were significantly lower in the partial nitritation system over the complete nitrification system. The abundance of Planctomycetes was higher in the partial nitritation process, indicating the anammox reaction occurred in the partial nitritation system. These results suggested the nitrite accumulation rate of circulating ratios 75% was the highest, with an average of 92%,and a possibility to treat the low Carbon Nitrogen ratio wastewater using the partial nitritation/anammox process.

SUBMITTER: Zhao Z

PROVIDER: S-EPMC6113234 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal.

Zhao Zhirui Z Luo Jinxue J Jin Bo B Zhang Jiayao J Li Bin B Ma Bin B An Xiaoyu X Zhang Shujun S Shan Baoqing B

Scientific reports 20180828 1

This work studied the microbial community in partial nitritation and complete nitrification processes, which were applied to treat the low Carbon Nitrogen ratio wastewater. The phospholipid fatty acid and quantitative PCR analysis showed that the sludge circulating ratio of 75% resulted in a good microbial growth and a higher abundance of ammonia oxidizing bacteria relative to the nitrite oxidizing bacteria. The Betaproteobacteria were observed to compose the most abundant sludge bacterial group ...[more]

PMID: 30154517

Similar Datasets

Project description:The emergence and spread of antibiotics resistance in wastewater treatment systems have been pointed as a major environmental health problem. Nevertheless, research about adaptation and antibiotics resistance gain in wastewater treatment systems subjected to antibiotics has not been successfully developed considering bioreactor performance, microbial community dynamics and microbial activity dynamics at the same time. To observe this in autotrophic nitrogen removal systems, a partial-nitritation biofilter was subjected to a continuous loading of antibiotics mix of azithromycin, norfloxacin, trimethoprim, and sulfamethoxazole. The effect of the antibiotics mix over the performance, bacterial communities and bacterial activity in the system was evaluated. The addition of antibiotics caused a drop of ammonium oxidation efficiency (from 50 to 5%) and of biomass concentration in the bioreactor, which was coupled to the loss of ammonium oxidizing bacteria Nitrosomonas in the bacterial community from 40 to 3%. Biomass in the partial nitritation biofilter experienced a sharp decrease of about 80% due to antibiotics loading, but the biomass adapted and experienced a growth by stabilization under antibiotics feeding. During the experiment several bacterial genera appeared, such as Alcaligenes, Paracoccus, and Acidovorax, clearly dominating the bacterial community with >20% relative abundance. The system reached around 30% ammonium oxidation efficiency after adaptation to antibiotics, but no effluent nitrite was found, suggesting that dominant antibiotics-resistant phylotypes could be involved in nitrification-denitrification metabolisms. The activity of ammonium oxidation measured as amoA and hao gene expression dropped a 98.25% and 99.21%, respectively, comparing the system before and after the addition of antibiotics. On the other hand, denitrifying activity increased as observed by higher expression of nir and nos genes (83.14% and 252.54%, respectively). In addition, heterotrophic nitrification cyt c-551 was active only after the antibiotics addition. Resistance to the antibiotics was presumably given by ermF, carA and msrA for azithromycin, mutations of the gyrA and grlB for norfloxacin, and by sul123 genes for sulfamethoxazole. Joined physicochemical and microbiological characterization of the system were used to investigate the effect of the antibiotics over the bioprocess. Despite the antibiotics resistance, activity of Bacteria decreased while the activity of Archaea and Fungi increased.

Dataset Information

Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal.

Publications

Analysis of Bacterial Communities in Partial Nitritation and Conventional Nitrification Systems for Nitrogen Removal.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets