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Pluripotent Stem Cell Platforms for Drug Discovery.

ABSTRACT: Use of human pluripotent stem cells (hPSCs) and their differentiated derivatives have led to recent proof-of-principle drug discoveries, defining a pathway to the implementation of hPSC-based drug discovery (hPDD). Current hPDD strategies, however, have inevitable conceptual biases and technological limitations, including the dimensionality of cell-culture methods, cell maturity and functionality, experimental variability, and data reproducibility. In this review, we dissect representative hPDD systems via analysis of hPSC-based 2D-monolayers, 3D culture, and organoids. We discuss mechanisms of drug discovery and drug repurposing, and roles of membrane drug transporters in tissue maturation and hPDD using the example of drugs that target various mutations of CFTR, the cystic fibrosis transmembrane conductance regulator gene, in patients with cystic fibrosis.

SUBMITTER: Chen KG

PROVIDER: S-EPMC6117164 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Pluripotent Stem Cell Platforms for Drug Discovery.

Chen Kevin G KG Mallon Barbara S BS Park Kyeyoon K Robey Pamela G PG McKay Ronald D G RDG Gottesman Michael M MM Zheng Wei W

Trends in molecular medicine 20180711 9

Use of human pluripotent stem cells (hPSCs) and their differentiated derivatives have led to recent proof-of-principle drug discoveries, defining a pathway to the implementation of hPSC-based drug discovery (hPDD). Current hPDD strategies, however, have inevitable conceptual biases and technological limitations, including the dimensionality of cell-culture methods, cell maturity and functionality, experimental variability, and data reproducibility. In this review, we dissect representative hPDD ...[more]

PMID: 30006147

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