Ontology highlight
ABSTRACT:
SUBMITTER: Klebl DP
PROVIDER: S-EPMC7137104 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Klebl David P DP Monteiro Diana C F DCF Kontziampasis Dimitrios D Kopf Florian F Sobott Frank F White Howard D HD Trebbin Martin M Muench Stephen P SP
Acta crystallographica. Section D, Structural biology 20200325 Pt 4
Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which has been used to achieve high resolution, particularly for well behaved samples such as apoferritin. However, this approach is flawed since the blotting process can have adverse effects on some proteins and ...[more]