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Biocompatible/Biodegradable Electrowetting on Dielectric Microfluidic Chips with Fluorinated CTA/PLGA.

ABSTRACT: One of the major hurdles in the development of biocompatible/biodegradable EWOD (Electrowetting-on-dielectric) devices is the biocompatibility of the dielectric and hydrophobic layers. In this study, we address this problem by using reactive ion etching (RIE) to prepare a super-hydrophobic film combining fluorinated cellulose triacetate (CTA) and poly (lactic-co-glycolic acid) (PLGA). The contact angle (CA) of water droplets on the proposed material is about 160°. X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) characterizations indicate that a slight increase in the surface roughness and the formation of CF_x (C-F or CF?) bonds are responsible for the super-hydrophobic nature of the film. Alternating Current (AC) static electrowetting and droplet transportation experiments evidence that contact angle hysteresis and contact line pinning are greatly reduced by impregnating the CTA/PLGA film with silicon oil. Therefore, this improved film could provide a biocompatible alternative to the typical Teflon^® or Cytop^® films as a dielectric and hydrophobic layer.

SUBMITTER: Zhang K

PROVIDER: S-EPMC6120052 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Biocompatible/Biodegradable Electrowetting on Dielectric Microfluidic Chips with Fluorinated CTA/PLGA.

Zhang Kaidi K Chao Lei L Zhou Jia J

Materials (Basel, Switzerland) 20180801 8

One of the major hurdles in the development of biocompatible/biodegradable EWOD (Electrowetting-on-dielectric) devices is the biocompatibility of the dielectric and hydrophobic layers. In this study, we address this problem by using reactive ion etching (RIE) to prepare a super-hydrophobic film combining fluorinated cellulose triacetate (CTA) and poly (lactic-co-glycolic acid) (PLGA). The contact angle (CA) of water droplets on the proposed material is about 160°. X-ray photoelectron spectroscop ...[more]

PMID: 30071631

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Project description:Access to diverse PET tracers for preclinical and clinical research remains a major obstacle to research in cancer and other disease research. The prohibitive cost and limited availability of tracers could be alleviated by microfluidic radiosynthesis technologies combined with a high-yield microscale radiosynthetic method. In this report, we demonstrate the multistep synthesis of 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) with high yield on an electrowetting-on-dielectric (EWOD) microfluidic radiosynthesizer, previously developed in our group. We have identified and established several parameters that are most critical in the microscale radiosynthesis, such as the reaction time, reagent concentration, and molar ratios, to successfully synthesize (18)F-FLT in this compact platform.(18)F-FLT was synthesized from the 3-N-Boc-1-[5-O-(4,4'-dimethoxytrityl)-3-O-nosyl-2-deoxy-?-D-lyxofuranosyl] thymine precursor on the EWOD chip starting from the first solvent exchange and (18)F-fluoride ion activation step to the final deprotection step. The fluorination reaction was performed in a mixture of thexyl alcohol and dimethyl sulfoxide. The crude product after deprotection was collected from the chip and purified on a custom-made solid-phase extraction cartridge and subjected to quality control testing. The purified (18)F-FLT was suitable for small-animal PET studies in multiple nude mice xenografted with the A431 carcinoma cell line.(18)F-FLT was successfully synthesized on the EWOD microdevice coupled with an off-chip solid-phase extraction purification with a decayed-corrected radiochemical yield of 63% ± 5% (n = 5) and passed all of the quality control tests required by the U.S. Pharmacopeia for radiotracers to be injected into humans. We have successfully demonstrated the synthesis of several batches of (18)F-FLT on EWOD, starting with approximately 333 MBq of radioactivity and obtained up to 52 MBq (non-decay-corrected) of (18)F-FLT on cartridge purification. The specific activity of 2 representative preparations of (18)F-FLT synthesized on the EWOD chip were measured to be 1,800 and 2,400 GBq/?mol.The EWOD microchip and optimized synthesis method in combination represent an effective platform for synthesizing (18)F-FLT with high yield and of good quality for imaging. This compact platform, with configurable synthesis steps, could potentially form the basis of a stand-alone system that decouples PET probe production from the cyclotron and specialized radiochemistry facilities and increases diversity and flexibility in probe production.

Dataset Information

Biocompatible/Biodegradable Electrowetting on Dielectric Microfluidic Chips with Fluorinated CTA/PLGA.

Publications

Biocompatible/Biodegradable Electrowetting on Dielectric Microfluidic Chips with Fluorinated CTA/PLGA.

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