Dataset Information

Electrowetting Using a Microfluidic Kelvin Water Dropper.

ABSTRACT: The Kelvin water dropper is an electrostatic generator that can generate high voltage electricity through water dripping. A conventional Kelvin water dropper converts the gravitational potential energy of water into electricity. Due to its low current output, Kelvin water droppers can only be used in limited cases that demand high voltage. In the present study, microfluidic Kelvin water droppers (MKWDs) were built in house to demonstrate a low-cost but accurately controlled miniature device for high voltage generation. The performance of the MKWDs was characterized using different channel diameters and flow rates. The best performed MKWD was then used to conduct experiments of the electrowetting of liquid on dielectric surfaces. Electrowetting is a process that has been widely used in manipulating the wetting properties of a surface using an external electric field. Usually electrowetting requires an expensive DC power supply that outputs high voltage. However, in this research, it was demonstrated that electrowetting can be conducted by simply using an MKWD. Additionally, an analytic model was developed to simulate the electrowetting process. Finally, the model's ability to well predict the liquid deformation during electrowetting using MKWDs was validated.

SUBMITTER: Yazdanshenas E

PROVIDER: S-EPMC6187503 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Electrowetting Using a Microfluidic Kelvin Water Dropper.

Yazdanshenas Elias E Tang Qiang Q Zhang Xiaoyu X

Micromachines 20180225 3

The Kelvin water dropper is an electrostatic generator that can generate high voltage electricity through water dripping. A conventional Kelvin water dropper converts the gravitational potential energy of water into electricity. Due to its low current output, Kelvin water droppers can only be used in limited cases that demand high voltage. In the present study, microfluidic Kelvin water droppers (MKWDs) were built in house to demonstrate a low-cost but accurately controlled miniature device for ...[more]

PMID: 30424026

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Project description:Access to diverse PET tracers for preclinical and clinical research remains a major obstacle to research in cancer and other disease research. The prohibitive cost and limited availability of tracers could be alleviated by microfluidic radiosynthesis technologies combined with a high-yield microscale radiosynthetic method. In this report, we demonstrate the multistep synthesis of 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT) with high yield on an electrowetting-on-dielectric (EWOD) microfluidic radiosynthesizer, previously developed in our group. We have identified and established several parameters that are most critical in the microscale radiosynthesis, such as the reaction time, reagent concentration, and molar ratios, to successfully synthesize (18)F-FLT in this compact platform.(18)F-FLT was synthesized from the 3-N-Boc-1-[5-O-(4,4'-dimethoxytrityl)-3-O-nosyl-2-deoxy-?-D-lyxofuranosyl] thymine precursor on the EWOD chip starting from the first solvent exchange and (18)F-fluoride ion activation step to the final deprotection step. The fluorination reaction was performed in a mixture of thexyl alcohol and dimethyl sulfoxide. The crude product after deprotection was collected from the chip and purified on a custom-made solid-phase extraction cartridge and subjected to quality control testing. The purified (18)F-FLT was suitable for small-animal PET studies in multiple nude mice xenografted with the A431 carcinoma cell line.(18)F-FLT was successfully synthesized on the EWOD microdevice coupled with an off-chip solid-phase extraction purification with a decayed-corrected radiochemical yield of 63% ± 5% (n = 5) and passed all of the quality control tests required by the U.S. Pharmacopeia for radiotracers to be injected into humans. We have successfully demonstrated the synthesis of several batches of (18)F-FLT on EWOD, starting with approximately 333 MBq of radioactivity and obtained up to 52 MBq (non-decay-corrected) of (18)F-FLT on cartridge purification. The specific activity of 2 representative preparations of (18)F-FLT synthesized on the EWOD chip were measured to be 1,800 and 2,400 GBq/?mol.The EWOD microchip and optimized synthesis method in combination represent an effective platform for synthesizing (18)F-FLT with high yield and of good quality for imaging. This compact platform, with configurable synthesis steps, could potentially form the basis of a stand-alone system that decouples PET probe production from the cyclotron and specialized radiochemistry facilities and increases diversity and flexibility in probe production.

Dataset Information

Electrowetting Using a Microfluidic Kelvin Water Dropper.

Publications

Electrowetting Using a Microfluidic Kelvin Water Dropper.

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