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Decreasing miRNA sequencing bias using a single adapter and circularization approach.

ABSTRACT: The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.

SUBMITTER: Barberan-Soler S

PROVIDER: S-EPMC6120088 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Decreasing miRNA sequencing bias using a single adapter and circularization approach.

Barberán-Soler Sergio S Vo Jenny M JM Hogans Ryan E RE Dallas Anne A Johnston Brian H BH Kazakov Sergei A SA

Genome biology 20180903 1

The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variet ...[more]

PMID: 30173660

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