The GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization.

ABSTRACT: In the cell, ?-tubulin establishes a cellular network of threads named the ?-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that ?-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that ?-tubulin has a role in the maintenance of the mitochondrial network and functions as reduced levels of ?-tubulin or impairment of its GTPase domain disrupts the mitochondrial network and alters both their respiratory capacity and the expression of mitochondrial-related genes. By contrast, reduced mitochondrial number or increased protein levels of ?-tubulin DNA-binding domain enhanced the association of ?-tubulin with mitochondria. Our results demonstrate that ?-tubulin is an important mitochondrial structural component that maintains the mitochondrial network, providing mitochondria with a cellular infrastructure. We propose that ?-tubulin provides a cytoskeletal element that gives form to the mitochondrial network.

SUBMITTER: Lindstrom L 

PROVIDER: S-EPMC6123723 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature