Stability of blood-based biomarkers of Alzheimer's disease over multiple freeze-thaw cycles.

ABSTRACT:

Introduction

Freeze-thaw instability may contribute to preanalytical variation in blood-based biomarker studies. We investigated the effects of up to four freeze-thaw cycles on single molecule array immunoassays of serum neurofilament light chain and plasma total tau, amyloid β 1-40 (Aß₄₀), and Aβ 1-42 (Aβ₄₂).

Methods

Individuals who had peripheral venepuncture during investigation of suspected neurodegenerative disease were recruited. After standardized preprocessing, 200 μL of plasma and serum aliquots were stored at -80°C within 60 minutes. Aliquots underwent one to four freeze-thaw cycles.

Results

There was no significant difference across four freeze-thaw cycles for serum neurofilament light chain (n = 12), plasma total tau (n = 11), or plasma Aβ₄₂ (n = 12). For plasma Aβ₄₀ (n = 14), there were significant median reductions by ratios of .96 and .92 at the third and fourth cycles, respectively.

Discussion

Up to four freeze-thaw cycles do not influence single molecule array blood biomarkers of neurofilament light chain, total tau, or Aβ₄₂, with at most minor reductions in Aβ₄₀.

SUBMITTER: Keshavan A 

PROVIDER: S-EPMC6131984 | biostudies-literature |

REPOSITORIES: biostudies-literature