Ontology highlight
ABSTRACT:
SUBMITTER: McClanahan PD
PROVIDER: S-EPMC7718750 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
McClanahan Patrick D PD McCloskey Richard J RJ Ng Tung Hing Melanie M Raizen David M DM Fang-Yen Christopher C
G3 (Bethesda, Md.) 20201203 12
Ultracold preservation is widely used for storage of genetic stocks of <i>Caenorhabditis elegans</i> Current cryopreservation protocols are vulnerable to refrigeration failures, which can result in the loss of stock viability due to damage during re-freezing. Here we present a method for preserving worms in a dehydrated and frozen form that retains viability after multiple freeze-thaw cycles. After dehydration in the presence of trehalose or glycerol, <i>C. elegans</i> stocks can be frozen and t ...[more]