Dataset Information

Naphthol Blue Black and ^99mTc-Labeled Mannosylated Human Serum Albumin (^99mTc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier.

ABSTRACT: ^99mTc-labeled mannosylated human serum albumin (MSA) has been reported as a sentinel lymph node (SLN)-imaging agent by binding to macrophages in the LNs. By conjugating it with blue dye, we developed a new multimodal radio-nanocarrier by visual investigation, fluorescence imaging, and single photon emission computed tomography (SPECT)/computed tomography (CT). Binding affinities of seven blue dyes to MSA were tested. According to the spectroscopic study and visual inspection of MSA-bound dyes, naphthol blue black (NBB) was selected as the best candidate of multimodal agent. Thus, ^99mTc-MSA-NBB conjugate was prepared and further investigated using mice. After footpad injection, it showed high popliteal LN accumulation at 1?h. SPECT/CT also showed high popliteal as well as inguinal LN uptakes at 10?min that sustained until 2?h. In conclusion, we prepared a multimodal SLN imaging radio-nanocarrier, ^99mTc-MSA-NBB conjugate, and confirmed its excellency as a multimodal probe for SLN mapping.

SUBMITTER: Lee JY

PROVIDER: S-EPMC6134003 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Naphthol Blue Black and 99mTc-Labeled Mannosylated Human Serum Albumin (99mTc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier.

Lee Ji Youn JY Kim Ho Young HY Lee Yun-Sang YS Jeong Jae Min JM

Scientific reports 20180911 1

99mTc-labeled mannosylated human serum albumin (MSA) has been reported as a sentinel lymph node (SLN)-imaging agent by binding to macrophages in the LNs. By conjugating it with blue dye, we developed a new multimodal radio-nanocarrier by visual investigation, fluorescence imaging, and single photon emission computed tomography (SPECT)/computed tomography (CT). Binding affinities of seven blue dyes to MSA were tested. According to the spectroscopic study and visual inspection of MSA-bo ...[more]

PMID: 30206287

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Dataset Information

Naphthol Blue Black and ^99mTc-Labeled Mannosylated Human Serum Albumin (^99mTc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier.

Publications

Naphthol Blue Black and <sup>99m</sup>Tc-Labeled Mannosylated Human Serum Albumin (<sup>99m</sup>Tc-MSA) Conjugate as a Multimodal Lymph Node Mapping Nanocarrier.

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