Protein fishing from single live cells.
Ontology highlight
ABSTRACT:
SUBMITTER: Shekaramiz E
PROVIDER: S-EPMC6134770 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
Protein fishing from single live cells.
Shekaramiz Elaheh E Doshi Rupak R Wickramasinghe H Kumar HK
Journal of nanobiotechnology 20180911 1
Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, ...[more]
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