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Protein fishing from single live cells.

ABSTRACT: Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.

SUBMITTER: Shekaramiz E

PROVIDER: S-EPMC6134770 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Protein fishing from single live cells.

Shekaramiz Elaheh E Doshi Rupak R Wickramasinghe H Kumar HK

Journal of nanobiotechnology 20180911 1

Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, ...[more]

PMID: 30205820

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