Dataset Information

Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.

ABSTRACT: Polymer-based multilayer microencapsulation technology represents one of the promising strategies for intracellular drug delivery, however, membrane processes involved in vehicle internalisation are not fully understood. Here we employed a scanning probe microscopy technique called Scanning Ion Conductance Microscopy (SICM) to study these complex processes at nanoscale resolution in real time. We were able to image topography simultaneously with local elastic modulus throughout the whole course of microcapsule internalisation in A549 cell culture without disrupting the internalisation process. The imaging revealed that capsules triggered the formation of membrane protrusions in their vicinity, which is an important but not a sufficient step towards full capsule internalisation. A crucial aspect appeared to be nanoscale restructuring of these protrusions into smooth thin layers extending over the surface of capsules. Simultaneous mapping of elastic modulus during capsule internalisation allowed monitoring the structural changes during extension of the membrane sheets over the surface of the capsule and the subsequent post-internalisation phenomenon of capsule buckling. To our knowledge these are the first experimental data capturing the interactions between the cellular membrane and microcapsules in their whole complexity with nanoscale resolution. The methodology established here has the potential to provide new insights into interactions at the interface between the nanostructured materials and cellular membrane under physiological conditions.

SUBMITTER: Chen Y

PROVIDER: S-EPMC6137606 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.

Chen Yuxiu Y Sukhorukov Gleb B GB Novak Pavel P

Nanoscale 20180901 35

Polymer-based multilayer microencapsulation technology represents one of the promising strategies for intracellular drug delivery, however, membrane processes involved in vehicle internalisation are not fully understood. Here we employed a scanning probe microscopy technique called Scanning Ion Conductance Microscopy (SICM) to study these complex processes at nanoscale resolution in real time. We were able to image topography simultaneously with local elastic modulus throughout the whole course ...[more]

PMID: 30176032

Similar Datasets

Project description:Polyelectrolyte microcapsules are among the most promising carriers of various sensing substances for their application inside the bloodstream of vertebrates. The long-term effects of biodegradable microcapsules in mammals are relatively well studied, but this is not the case for non-biodegradable microcapsules, which may be even more generally applicable for physiological measurements. In the current study, we introduced non-biodegradable polyelectrolyte microcapsules coated with polyethylene glycol (PMs-PEG) into the circulatory system of zebrafish to assess their long-term effects on fish internal organs with histopathologic analysis. Implantation of PMs-PEG was not associated with the formation of microclots or thrombi in thin capillaries; thus, the applied microcapsules had a low aggregation capacity. The progression of the immune response to the implant depended on the time and the abundance of microparticles in the tissues. We showed that inflammation originated from recognition and internalization of PMs-PEG by phagocytes. These microcapsule-filled immune cells have been found to migrate through the intestinal wall into the lumen, demonstrating a possible mechanism for partial microparticle elimination from fish. The observed tissue immune response to PMs-PEG was local, without a systemic effect on the fish morphology. The most pronounced chronic severe inflammatory reaction was observed near the injection site in renal parenchyma and within the abdominal cavity since PMs-PEG were administered with kidney injection. Blood clots and granulomatosis were noted at the injection site but were not found in the kidneys outside the injection site. Single microcapsules brought by blood into distal organs did not have a noticeable effect on the surrounding tissues. The severity of noted pathologies of the gills was insufficient to affect respiration. No statistically significant alterations in hepatic morphology were revealed after PMs-PEG introduction into fish body. Overall, our data demonstrate that despite they are immunogenic, non-biodegradable PMs-PEG have low potential to cause systemic effects if applied in the minimal amount necessary for detection of fluorescent signal from the microcapsules.

Dataset Information

Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.

Publications

Visualising nanoscale restructuring of a cellular membrane triggered by polyelectrolyte microcapsules.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets