High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner.

ABSTRACT: The Frontier Microfocus Macromolecular Crystallography (FMX) beamline at the National Synchrotron Light Source II with its 1 µm beam size and photon flux of 3 × 10¹² photons s^-1 at a photon energy of 12.66 keV has reached unprecedented dose rates for a structural biology beamline. The high dose rate presents a great advantage for serial microcrystallography in cutting measurement time from hours to minutes. To provide the instrumentation basis for such measurements at the full flux of the FMX beamline, a high-speed, high-precision goniometer based on a unique XYZ piezo positioner has been designed and constructed. The piezo-based goniometer is able to achieve sub-100 nm raster-scanning precision at over 10 grid-linepairs s^-1 frequency for fly scans of a 200

SUBMITTER: Gao Y 

PROVIDER: S-EPMC6140394 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature