Ontology highlight
ABSTRACT:
SUBMITTER: Kraatz SHW
PROVIDER: S-EPMC6146289 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Kraatz Sebastian H W SHW Bianchi Sarah S Steinmetz Michel O MO
Bioscience reports 20180919 5
Coiled-coils are ubiquitous protein-protein interaction motifs found in many eukaryotic proteins. The elongated, flexible and often irregular nature of coiled-coils together with their tendency to form fibrous arrangements in crystals imposes challenges on solving the phase problem by molecular replacement. Here, we report the successful combinatorial use of native and rational engineered disulfide bridges together with sulfur-SAD phasing as a powerful tool to stabilize and solve the structure o ...[more]