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3'-End labeling of nucleic acids by a polymerase ribozyme.

ABSTRACT: A polymerase ribozyme can be used to label the 3' end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.

SUBMITTER: Samanta B

PROVIDER: S-EPMC6158495 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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3'-End labeling of nucleic acids by a polymerase ribozyme.

Samanta Biswajit B Horning David P DP Joyce Gerald F GF

Nucleic acids research 20180901 17

A polymerase ribozyme can be used to label the 3' end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs ...[more]

PMID: 29901762

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