Ontology highlight
ABSTRACT:
SUBMITTER: Kouba T
PROVIDER: S-EPMC7749160 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Kouba Tomáš T Koval' Tomáš T Sudzinová Petra P Pospíšil Jiří J Brezovská Barbora B Hnilicová Jarmila J Šanderová Hana H Janoušková Martina M Šiková Michaela M Halada Petr P Sýkora Michal M Barvík Ivan I Nováček Jiří J Trundová Mária M Dušková Jarmila J Skálová Tereza T Chon URee U Murakami Katsuhiko S KS Dohnálek Jan J Krásný Libor L
Nature communications 20201218 1
RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors for recovery from stalled states and adaptation to environmental changes. Here, we investigated the mechanism by which a helicase-like factor HelD recycles RNAP. We report a cryo-EM structure of a complex between the Mycobacterium smegmatis RNAP and HelD. The crescent-shaped HelD simultaneously penetrates deep into two RNAP channels that are responsible for nucleic acids binding and substrate delivery to the ...[more]