A GTP-synthesizing ribozyme selected by metabolic coupling to an RNA polymerase ribozyme.

ABSTRACT: Synthesis of RNA in early life forms required chemically activated nucleotides, perhaps in the same form of nucleoside 5′-triphosphates (NTPs) as in the contemporary biosphere. We show the development of a catalytic RNA (ribozyme) that generates the nucleoside triphosphate guanosine 5′-triphosphate (GTP) from the nucleoside guanosine and the prebiotically plausible cyclic trimetaphosphate. Ribozymes were selected from 1.6 × 10¹⁴ different randomized sequences by metabolically coupling 6-thio GTP synthesis to primer extension by an RNA polymerase ribozyme within 10¹⁶ emulsion droplets. Several functional RNAs were identified, one of which was characterized in more detail. Under optimized reaction conditions, this ribozyme produced GTP at a rate 18,000-fold higher than

SUBMITTER: Akoopie A 

PROVIDER: S-EPMC8494290 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature