Ontology highlight
ABSTRACT:
SUBMITTER: Nixon FM
PROVIDER: S-EPMC6173286 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Nixon Faye M FM Honnor Thomas R TR Clarke Nicholas I NI Starling Georgina P GP Beckett Alison J AJ Johansen Adam M AM Brettschneider Julia A JA Prior Ian A IA Royle Stephen J SJ
Journal of cell science 20170407 10
Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle in human cells. We present four examples of uses for this workflow that are not practical by light microscopy and/or transmission electron microscopy. First, distinguishing closely associated microtubules within K-fibers; second, resolving bridging ...[more]