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Multicolor single-particle reconstruction of protein complexes.

ABSTRACT: Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.

SUBMITTER: Sieben C

PROVIDER: S-EPMC6173288 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Multicolor single-particle reconstruction of protein complexes.

Sieben Christian C Banterle Niccolò N Douglass Kyle M KM Gönczy Pierre P Manley Suliana S

Nature methods 20181001 10

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, ...[more]

PMID: 30275574

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