Dataset Information

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors.

ABSTRACT: Conductive materials facilitate direct interspecies electron transfer between acidogens and methanogens during methane (CH₄) production. Soluble microbial products (SMP) and extracellular polymeric substances (EPS) produced by microorganisms might act as the electron shuttle between microorganisms and conductive materials. In this study, effects of conductive ferroferric oxide (Fe₃O₄) on anaerobic treatment process and the production of SMP and EPS were investigated. The maximum CH₄ production rate was enhanced by 23.3% with the dosage of Fe₃O₄. The concentrations of proteins, polysaccharides, and humic substances in tightly bound EPS (T-EPS) were promoted, suggesting that extracellular metabolisms were induced by conductive materials. Distribution of potential electron shuttles such as quinone-like substances, flavins, aromatic amino acids, and dipeptides in SMP and EPS phases were comprehensively investigated and these electron shuttles were significantly affected by Fe₃O₄. Dipeptides consisting of phenylalanine were widely detected in T-EPS of the Fe₃O₄ reactor, indicating a potential different extracellular electron exchange pattern with the addition of conductive materials.

SUBMITTER: Yin Q

PROVIDER: S-EPMC6189335 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors.

Yin Qidong Q He Kai K Echigo Shinya S Wu Guangxue G Zhan Xinmin X Hu Hongying H

Frontiers in microbiology 20181009

Conductive materials facilitate direct interspecies electron transfer between acidogens and methanogens during methane (CH4) production. Soluble microbial products (SMP) and extracellular polymeric substances (EPS) produced by microorganisms might act as the electron shuttle between microorganisms and conductive materials. In this study, effects of conductive ferroferric oxide (Fe3O4) on anaerobic treatment process and the production of SMP and EPS were investiga ...[more]

PMID: 30356728

Similar Datasets

Project description:Ammonia (NH3) inhibition represents a major limitation to methane production during anaerobic digestion of organic material in biogas reactors. This process relies on co-operative metabolic interactions between diverse taxa at the community-scale. Despite this, most investigations have focused singularly on how methanogenic Archaea respond to NH3 stress. With a high-NH3 pre-adapted and un-adapted community, this study investigated responses to NH3 inhibition both at the community-scale and down to individual taxa. The pre-adapted community performed methanogenesis under inhibitory NH3 concentrations better than the un-adapted. While many functionally important phyla were shared between the two communities, only taxa from the pre-adapted community were robust to NH3. Functionally important phyla were mostly comprised of sensitive taxa (≥ 50%), yet all groups, including methanogens, also possessed tolerant individuals (10-50%) suggesting that potential mechanisms for tolerance are non-specific and widespread. Hidden Markov Model-based phylogenetic analysis of methanogens confirmed that NH3 tolerance was not restricted to specific taxonomic groups, even at the genus level. By reconstructing covarying growth patterns via network analyses, methanogenesis by the pre-adapted community was best explained by continued metabolic interactions (edges) between tolerant methanogens and other tolerant taxa (nodes). However, under non-inhibitory conditions, sensitive taxa re-emerged to dominate the pre-adapted community, suggesting that mechanisms of NH3 tolerance can be disadvantageous to fitness without selection pressure. This study demonstrates that methanogenesis under NH3 inhibition depends on broad-scale tolerance throughout the prokaryotic community. Mechanisms for tolerance seem widespread and non-specific, which has practical significance for the development of robust methanogenic biogas communities. KEY POINTS: • Ammonia pre-adaptation allows for better methanogenesis under inhibitory conditions. • All functionally important prokaryote phyla have some ammonia tolerant individuals. • Methanogenesis was likely dependent on interactions between tolerant individuals.

Dataset Information

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors.

Publications

Ferroferric Oxide Significantly Affected Production of Soluble Microbial Products and Extracellular Polymeric Substances in Anaerobic Methanogenesis Reactors.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets