Ontology highlight
ABSTRACT:
SUBMITTER: Ptacek J
PROVIDER: S-EPMC6194262 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Ptacek Jakub J Nedvedova Jana J Navratil Michal M Havlinova Barbora B Konvalinka Jan J Barinka Cyril C
Protein science : a publication of the Protein Society 20180901 9
Calcium ions are required for proper function of a wide spectrum of proteins within cells. X-ray crystallography of human glutamate carboxypeptidase II (GCPII) revealed the presence of a Ca<sup>2+</sup> -binding site, but its importance for the structure and function of this metallopeptidase has not been elucidated to date. Here, we prepared a panel of mutants targeting residues that form the Ca<sup>2+</sup> coordination sphere of GCPII and analyzed their structural and enzymatic properties usin ...[more]