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Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130?nm.


ABSTRACT: High-resolution optical microscopes that can break 180?nm in spatial resolution set to conventional microscopies are much-needed tools. However, current optical microscopes have to rely on exogenous fluorescent labels to achieve high resolution in biological imaging. Herein, we report near-resonance enhanced label-free stimulated Raman scattering (SRS) microscopy with a lateral resolution near 130?nm, in which the high-resolution image contrast originates directly from a low concentration of endogenous biomolecules, with sensitivity gains of approximately 23 times. Moreover, by using a 0.3-m-long optical fiber, we developed hyperspectral SRS microscopy based on spectral focusing technology. Attributed to enhancements in spatial resolution and sensitivity, we demonstrated high-resolution imaging of three-dimensional structures in single cells and high-resolution mapping of large-scale intact mouse brain tissues in situ. By using enhanced high-resolution hyperspectral SRS, we chemically observed sphingomyelin distributed in the myelin sheath that insulates single axons. Our concept opens the door to biomedical imaging with ~130?nm resolution.

SUBMITTER: Bi Y 

PROVIDER: S-EPMC6199294 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm.

Bi Yali Y   Yang Chi C   Chen Yage Y   Yan Shuai S   Yang Guang G   Wu Yaozu Y   Zhang Guoping G   Wang Ping P  

Light, science & applications 20181024


High-resolution optical microscopes that can break 180 nm in spatial resolution set to conventional microscopies are much-needed tools. However, current optical microscopes have to rely on exogenous fluorescent labels to achieve high resolution in biological imaging. Herein, we report near-resonance enhanced label-free stimulated Raman scattering (SRS) microscopy with a lateral resolution near 130 nm, in which the high-resolution image contrast originates directly from a low concentration of end  ...[more]

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