Ontology highlight
ABSTRACT:
SUBMITTER: Jiang M
PROVIDER: S-EPMC6206058 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Jiang Mengmeng M Chen Haide H Lai Shujing S Wang Renying R Qiu Yunfei Y Ye Fang F Fei Lijiang L Sun Huiyu H Xu Yang Y Jiang Xinyi X Zhou Ziming Z Zhang Tingyue T Li Yanwei Y Xie Jin J Fang Qun Q Gale Robert Peter RP Han Xiaoping X Huang He H Guo Guoji G
Cell discovery 20181030
Identification of effective culture conditions to maintain and possibly expand human HSPCs in vitro is an important goal. Recent advances highlight the efficacy of chemicals in maintaining and converting cell fates. We screened 186 chemicals and found that a combination of CHIR-99021, Forskolin and OAC1 (CFO) maintained human CD34-positive cells in vitro. Efficiency of the culture system was characterized using flow cytometry for CD34-positive cells, a colony-forming assay and xeno-transplants. ...[more]