Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems.

ABSTRACT:

Introduction

Specific immunotherapy is critical for alleviating symptoms associated with house dust mite allergy, such as asthma and rhinitis. However, this approach relies on crude extracts, which are often not of sufficient quality or purity and are not standardized. The use of recombinant allergens may enable safer, more effective treatment.

Material and methods

Using our previously constructed plasmids pET28a(+)-Der f 1, pET28a(+)-Der f 2 and pET28b(+)-Der f 4 as templates, the gene fragments coding for the allergens Der f 1, Der f 2 and Der f 4, respectively, of the dust mite Dermatophagoides farinae were amplified by PCR. Next the PCR-amplified DNAs were recovered, cloned into pFastBacHT A, and transformed into Escherichia coli DH10Bac. The resulting vect

SUBMITTER: Cui Y 

PROVIDER: S-EPMC6209719 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature