Ontology highlight
ABSTRACT:
SUBMITTER: Terranova L
PROVIDER: S-EPMC6214103 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Terranova Leonardo L Oriano Martina M Teri Antonio A Ruggiero Luca L Tafuro Camilla C Marchisio Paola P Gramegna Andrea A Gramegna Andrea A Contarini Martina M Franceschi Elisa E Sottotetti Samantha S Cariani Lisa L Bevivino Annamaria A Chalmers James D JD Aliberti Stefano S Blasi Francesco F
International journal of molecular sciences 20181020 10
Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of sputum. Sputum treatments with and without DTT were compared in terms of their median levels and the coefficient of variation between replicates of both DNA extraction yield and real-time PCR for the ...[more]